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ldh [2019-02-19 20:12:21]
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ldh [2019-02-19 20:12:21]

L-lactate dehydrogenase
Locus
BSU03050
Isoelectric point
5.39
Molecular weight
34.00 kDa
Protein length
321 aa Sequence Blast
Gene length
966 bp Sequence Blast
Function
overflow metabolism, fermentation
Product
L-lactate dehydrogenase
Essential
no
E.C.
1.1.1.27
Synonyms
lctE

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
329,774 330,739

The protein

Catalyzed reaction/ biological activity

  • (S)-lactate + NAD+ = pyruvate + NADH (according to Swiss-Prot)
  • Protein family

  • LDH family (according to Swiss-Prot)
  • Modification

  • phosphorylation on Tyr-224 PubMed
  • Structure

  • 3PQE
  • [PDB|A general discussion of Ldh structure]
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • Rex: repression, PubMed, in Rex regulon
  • Regulation

  • induced under anaerobic conditions (Rex) PubMed
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    Biological materials

    Mutant

  • BKE03050 (ldh::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAATCATCCTTCCAGGG, downstream forward: _UP4_TAACCGCAACTTTAGAGTAA
  • BKK03050 (ldh::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAATCATCCTTCCAGGG, downstream forward: _UP4_TAACCGCAACTTTAGAGTAA
  • GP2597 (ldh::tet comIQ12L) (in DK1042) available at Jrg Stlke's lab
  • References

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